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nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts)  (Lonza)


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    Lonza nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts)
    Nucleofector Tm Solution (Basic Nucleofector Tm Kit, Primary Fibroblasts), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofector+tm+solution+(basic+nucleofector+tm+kit%2C+primary+fibroblasts)/4d+nucleofector/pm29601977-91-13-19
    Average 90 stars, based on 1 article reviews
    nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts) - by Bioz Stars, 2026-09
    90/100 stars

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    TALENs:

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L + R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F + PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype.
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L+R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F+PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).

    Transfection:

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L + R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F + PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype.
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L+R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F+PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).

    Plasmid Preparation:

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L + R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F + PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).

    Article Title: SURF1 knockout cloned pigs: Early onset of a severe lethal phenotype.
    Article Snippet: .. For TALENs transfections, cells were trypsinized, counted and resuspended in Nucleofector TM solution (Basic Nucleofector TM Kit, Primary Fibroblasts, LONZA, Basel, Switzerland) supplemented with 2 μg of each TALEN arm (L+R) plasmid and 1 μg of HR vector (not used in transient transfection) (Supplementary methods). .. Recombinant colonies were screened by PCR for NHEJ-derived mutations using the S1TF and S1TR oligos giving a 375 bp product (Supplementary Table S3); for the integration of the HR vector the Ex2F+PuroF2-R oligos, amplifying a 1237 bp fragment, were used (Supplementary Table S3).



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    Lonza nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts)
    Nucleofector Tm Solution (Basic Nucleofector Tm Kit, Primary Fibroblasts), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofector+tm+solution+(basic+nucleofector+tm+kit%2C+primary+fibroblasts)/4d+nucleofector/pm29601977-91-13-19
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    nucleofector tm solution (basic nucleofector tm kit, primary fibroblasts) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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